Activity of membrane-associated sucrose synthase is regulated by its phosphorylation status in cultured cells of sycamore (Acer pseudoplatanus)
نویسندگان
چکیده
Agrobioteknologia eta Natura Baliabideetako Instituta, Consejo Superior de Investigaciones Cientı́ficas eta Nafarroako Unibertsitate Publikoa, Mutiloako etorbidea z/g, 31192 Mutiloabeti, Nafarroa, Spain University of Florida, IFAS, Department of Horticulture, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, 33850, USA *Corresponding author, e-mail: [email protected]
منابع مشابه
ADP-Glucose Transport by the Chloroplast Adenylate Translocator Is Linked to Starch Biosynthesis.
In organello starch biosynthesis was studied using intact chloroplasts isolated from spinach leaves (Spinacia oleracea). Immunoblot analysis using a specific antiserum against the mitochondrial adenylate (ADP/ATP) translocator of Neurospora crassa shows the presence of an adenylate translocator protein in the chloroplast envelope membranes, similar to that existing in mitochondria and amyloplas...
متن کاملProtein Phosphorylation in Amyloplasts Isolated from Suspension-Cultured Cells of Sycamore (Acer pseudoplatanus L.).
Highly purified amyloplasts were isolated from cultured cells of sycamore (Acer pseudoplatanus L.). Incubation of amyloplasts with [gamma-(32)P]-ATP resulted in the labeling of more than ten polypeptides. Pulsechase experiments showed the reversibility of the process with some but not all of the polypeptides. The phosphorylation reaction of one polypeptide, M(r) 100, was shown to be calcium dep...
متن کاملA novel sucrose synthase pathway for sucrose degradation in cultured sycamore cells.
Enzymes of sucrose degradation and glycolysis in cultured sycamore (Acer pseudoplatanus L.) cells were assayed and characterized in crude extracts and after partial purification, in an attempt to identify pathways for sucrose catabolism. Desalted cell extracts contained similar activities (20-40 nanomoles per milligram protein per minute) of sucrose synthase, neutral invertase, glucokinase, fru...
متن کاملArtifactual detection of ADP-dependent sucrose synthase in crude plant extracts.
Results presented in a previous report from this laboratory indicated the presence, in crude extracts from sycamore (Acer pseudoplatanus) and spinach (Spinacea oleracea), of a sucrose synthase (EC 2.4.1.13) showing high affinity for ADP as the glucose acceptor in the sucrose-cleaving reaction. In the present paper we report that the modified enzymatic method previously used to measure sucrose s...
متن کاملCharacterization and Intraorganellar Distribution of Protein Kinases in Amyloplasts Isolated from Cultured Cells of Sycamore (Acer pseudoplatanus L.).
Incubation of amyloplasts isolated from cultured cells of sycamore (Acer pseudoplatanus L.) with [gamma-(32)P]ATP resulted in the rapid phosphorylation (half-time of 40 seconds at 25 degrees Celcius) of organellar polypeptides. The preferred substrate for amyloplast protein kinases was Mg(2+). ATP, and recovery of only [(32)P]serine after partial acid hydrolysis indicated the predominance of pr...
متن کامل